Fig. 4.

Human islet miR-21 is increased by inflammatory cytokine treatment and increases apoptosis in association with reduced BCL2 expression. (a, b) miR-21 expression was quantified after human islets were treated with a cytokine mix for 24 and 48 h. (c) Cleaved caspase 3 was quantified using ELISA in dispersed human islets after miR-21 mimic transfection. A 48 h treatment with cytokine mix was also included as a positive control. (d) BCL2 transcripts were quantified after mimic transfection. Neg, negative. n=3–4. *p≤0.05