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Reduced to oxidized glutathione ratio analysis. Measurement of reduced to oxidized glutathione ratio (GSH/GSSG) was performed in C2C12 myotubes after 2, 6, or 24 h (T0–T24) of tadalafil (1 μM) treatments. Data presented are the mean ± SD of three experiments. *p < 0.05 vs T0
