Table 1.

Glutathione and total antioxidant capacity analysis

	T0	Tadalafil 1 μM
		T2	T6	T24
tGSHa	130.38 ± 1.88	146.56 ± 3.03#	158.78 ± 10.07#	149.41 ± 12.13
GSSGa	14.23 ± 1.26	17.09 ± 0.93*	21.18 ± 1.19#	17.10 ± 2.85
GSHa	116.14 ± 2.08	129.46 ± 2.13*	137.61 ± 10.08*	132.31 ± 9.60*
TACb	0.38 ± 0.07	0.39 ± 0.06	0.40 ± 0.02	0.41 ± 0.06

Measurement of total (tGSH), oxidized (GSSG), and reduced glutathione (GSH) and total antioxidant capacity (TAC) was performed in C2C12 myotubes after 2, 6, or 24 h (T0-T24) of tadalafil (1 μM) treatments. Data presented are the mean ± SD of three experiments

*p < 0.05 vs T0; #p < 0.01 vs T0

^anmol/mg protein

^bμmol Trolox/mg protein