Fig. 4.

Misfolded/oxidized proteins activate the heat shock proteins and ER chaperones in response to acute hypoxic stress. Graph shows the significant increase of heat shock proteins in acute hypoxic insult in muscle tissue homogenate. a HSP70. b HSP60. c Representative western blot identifying the expression of ER chaperone (GRP-78) in cytosolic tissue extract. Loading control was normalized with reference to GAPDH. d Graph represents the semiquantitative densitometric analysis of the protein expression. The net intensity was analyzed by using the ImageJ software. Data represents the mean ± SE; N = 5. Different symbols, double dagger, asterisk, and dagger indicate the significant differences between experimental groups (p < 0.05), while groups with matching symbols denote no difference (p ≥ 0.05)