Figure 4.

Impact of EGF stimulation on MDA-MB-231 breast cancer cells with (a–c) and without (d–f) overexpression of MenaINV. (a) Average 2D MSD of MenaINV overexpressing cells without EGF (blue) and with 5 nM EGF (red). Circles are data and curves are fits to Eq. 14. The R² values for the fits are 0.9986 for without EGF and 0.9956 for with EGF. (b) MSDs at t = 0.05 s without EGF (blue) (0.0076 ± 0.0004 μm², n = 157) and with EGF (red) (0.0116 ± 0.0005 μm², n = 162). (c) β at t = 1 s without EGF (blue) (0.37 ± 0.02, n = 157) and with EGF (red) (0.27 ± 0.02, n = 162). (d) Average 2D MSD of non-MenaINV overexpressing cells without EGF (blue) and with 5 nM EGF (red). Circles are data and curves are fits to Eq. 14. The R² values for the fits are 0.9994 for without EGF and 0.9992 for with EGF. (e) MSDs at t = 0.05 s without EGF (blue) (0.0085 ± 0.0004 μm², n =148) and with EGF (red) (0.0079 ± 0.0004 μm², n = 169). (f) β at t = 1 s without EGF (blue) (0.45 × 0.02, n =148) and with EGF (red) (0.43 ± 0.02, n = 169). Asterisk indicates p < 0.01, n is the number of particles tracked in each case, and error bars and values indicate mean ± SE.