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. 2017 May 9;112(9):1863–1873. doi: 10.1016/j.bpj.2017.03.037

Figure 2.

Figure 2

5-HT protects RBCs from cumene hydroperoxide-induced oxidation. (A) Shown here is the time course of the lipid peroxidation of RBCs labeled with the oxidation-sensitive probe BODIPY 581/591 C11 (BODIPY-RBC). CumOOH (150 μM) and 5-HT were added at t = 0 and the green/red fluorescence intensities were measured using flow cytometry. Inhibition of CumOOH-induced lipid peroxidation of BODIPY-RBC by 5-HT (B) or Mel (C) is given. BODIPY-RBC were preincubated for 30 min and then washed (●) before the CumOOH incubation or coincubated (▲) with the oxidant.