Figure 4.

The MAPK1-vinculin signaling complex is mechanosensitive. The results of the (A) MAPK1-open vinculin and (B) MAPK1-closed vinculin simulations are compared. Shown are energy plots of the D1-MAPK1, D2-MAPK1, D3-MAPK1, D4-MAPK1, PRL-MAPK1, Vt-MAPK1, and full-length vinculin-MAPK1 interaction energies. For the sake of clarity, error bars are only shown for the full-length vinculin-MAPK1 energy plots. Several of these energy plots overlap at 0 kcal/mol, and thus are not visible on the figure. These results demonstrate a clear difference in the strength of interaction between MAPK1 and closed versus open vinculin. To see this figure in color, go online.