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. 2017 May 9;112(9):1885–1893. doi: 10.1016/j.bpj.2017.03.039

Figure 5.

Figure 5

Changes in vinculin D3–D4 cleft size underlie mechanosensitivity. (A) Closed vinculin was superimposed onto the structure of open vinculin. Closed vinculin domains D3 and D4 are shown in light blue and light orange, respectively, and open vinculin domains D3 and D4 are shown in dark blue and dark orange, respectively. Closed vinculin residues 762–768 are highlighted in light purple, and open vinculin residues 762–768 are highlighted in dark purple. D3–D4 cleft size is shown, and was calculated by measuring the distance between residue 607 of D3 with 754 of D4. D3–D4 cleft size is considerably smaller in closed vinculin. (B) Closed vinculin was superimposed onto open vinculin from the structure of the open vinculin-MAPK1 complex. MAPK1 is shown in surface representation, and vinculin is shown in ribbon representation. The N-lobe (red) and C-lobe (yellow) of MAPK1, as well as the D3 (blue) and D4 (orange) domains of vinculin are shown. Indicated by arrows are the areas of overlap between MAPK1 from the MAPK1-open vinculin structure and closed vinculin. This indicates that the MAPK1 binding interface present on open vinculin is inaccessible to closed vinculin. To see this figure in color, go online.