Table 1.
Primers | Sequences |
---|---|
EcoRINSP7α-F (yeast two-hybrid) | TAATGAATTCTCGCTGACTGGTGCCCTC |
BamHINSP7α-R (yeast two-hybrid) | TAATGGATCCCTCCAGAACTTTCGGTGG |
BamHINSP7α-F (pull-down assay) | TAATGGATCCTCGCTGACTGGTGCCCTC |
XhoINSP7α-R (pull-down assay) | TAATCTCGAGTCACTCCAGAACTTTCGGT |
NSP7αK61A-F | TTGATGCGGTTCGAGGTACTTTG |
NSP7αK61A-R | TACCTCGAACCGCATCAACCTGCAC |
NSP7αL69A-F | GGCCAAAGCTGAAGCTTTTGCTGAT |
NSP7αL69A-R | AAGCTTCAGCTTTGGCCAAAGTACC |
NSP7αF72A-F | CTTGAAGCTGCTGCTGATACCGTG |
NSP7αF72A-R | ATCAGCAGCAGCTTCAAGTTTGGC |
Restriction enzyme sites are underlined.