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. 2017 May 11;8:541. doi: 10.3389/fimmu.2017.00541

Table 1.

Oligonucleotide primers used in RT-qPCR.

Primer Accession number Sequence (sense, antisense) RNA/DNA Tm°C
Rps9 NM_029767 Forward primer TTGTCGCAAAACCTATGTGACC 147/344 61.1
Reverse primer GCCGCCTTACGGATCTTGG 62.8
TNF-α X02611 Forward primer CCTGTAGCCCACGTCGTAG 148/442 61.5
Reverse primer GGGAGTAGACAAGGTACAACCC 61.4
HIF-1α NM_001313919.1 Forward primer CATCCAGAAGTTTTCTCACACG 138/– 63.5
Reverse primer GGCGAAGCAAAGAGTCTGAA 64.5