Figure 1.

TNFα and TGFβ1 modify private and shared transcriptional programs in MSCs. Human BM-derived MSCs of Donor #1 were stimulated by TNFα (50 ng/ml) or TGFβ1 (10 ng/ml) or treated by a vehicle control, as illustrated in the experimental design of Figure S1A in Supplementary Material (cytokine concentrations were selected as described in Section “Materials and Methods”; experiment performed in FBS-free medium). RNA was subjected to Illumina Beadchip array analyses, and the complete dataset was deposited at ArrayExpress [(51, 52); accession numbers E-MTAB-5421 and E-MTAB-5420]. (A) The figure presents the transcriptional programs modified in MSCs at different time points following their stimulation by TNFα or TGFβ1. Data are presented by p-value scaling (p ≤ 0.001 after Benjamini–Yekutieli correction for multiple testing). All the transcriptional programs that are demonstrated include ≥10 genes. (B) Venn diagram showing the number of transcriptional programs significantly affected only by TNFα, only by TGFβ1, or by both cytokines.