Figure 3.

qPCR validations of TNFα- or TGFβ1-upregulated mRNAs. Following MSC stimulation by TNFα or TGFβ1 and the global gene analysis described in previous figures, upregulation of 18 selected mRNAs was validated. All 18 mRNAs were modified in the original array analysis by log₂ fold change ≥ 1.5 (= fold change ≥ 2.8; * = fold change 2.6) with p ≤ 0.005 (after Benjamini–Hochberg correction for multiple testing, compared to vehicle-treated cells). The qPCR validations were performed with MSCs of two different donors (Donor #2 and Donor #3). The 18 selected mRNAs were validated by qPCR at time points in which they have undergone significant changes in the array analysis.