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Effects of HSYA on MMP-2, TIMP-1, and TIMP-2 expression in TGF-β1-activated MRC-5 cells. MRC-5 cells were pretreated with HSYA and/or 2 μmol/L SB431542 and then cultured with 10 ng/mL TGF-β1 for 72 h. Protein expression levels were measured by western blotting (A). Densitometric analyses of MMP-2 (B), TIMP-1 (C), and TIMP-2 (D). Data are presented as mean ± SD, n = 4 per group. Δp < 0.05 vs. normal group.
