FIGURE 4.

Effects of HSYA on α-SMA, COL1A1, and FN protein expression in TGF-β1-treated MRC-5 cells with TβRII knockdown. MRC-5 cells were pretreated with TβRII-non-targeting control siRNA or TβRII siRNA for 24 h followed by 45 μmol/L HSYA for 30 min before stimulation with 10 ng/mL TGF-β1 for 48 h. Protein expression levels of α-SMA, COL1A1, and FN were measured by western blotting (A). Densitometric analyses of α-SMA (B), COL1A1 (C), and FN (D). Data are presented as mean ± SD, n = 3 per group. ^ΔΔp < 0.01 vs. normal-untransfected group, ^▴p < 0.05, ^▴▴p < 0.01 vs. normal-non-targeting control siRNA group, ^∗p < 0.05, ^∗∗p < 0.01 vs. TGF-β1-untransfected group. ^##p < 0.01 vs. TGF-β1-non-targeting control siRNA group.