Figure 2.

PML Reduction Promotes an Epi-like Stem Cell State

(A) Heatmap with the top representative naive and primed genes.

(B) OTX2, CDH1, NANOG, and KLF4 protein levels prior to and after PML loss in ESC.

(C) Luciferase activity of BRE-Luc reporter upon PML depletion. Data represent the mean + SD of four independent experiments (n = 4). ^∗p < 0.05, ^∗∗p < 0.01.

(D) mRNA levels of BMP signaling target genes (Id1, Id2, Id3) in WT, PML KD, and KO ESC. Data are shown as mean + SD of three independent experiments (n = 3).

(E) CAGA-Luc reporter activity upon PML ablation. Error bars indicate +SD of four independent experiments (n = 4). ^∗p < 0.05.

(F) Heatmap of the gene expression profiles of our WT and PML KD ESC replicates along with three EpiSC and two ESC lines.