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. 2017 Apr 13;8(5):1164–1173. doi: 10.1016/j.stemcr.2017.03.014

Figure 1.

Figure 1

Primary and ASC-Adipocyte Integrity and Transcriptional Profiling

(A) Primary adipocytes isolated from whole adipose. Adipocytes were stained with DAPI to visualize the DNA (blue), BoDIPY to stain the lipid droplet (green), and LavaCell to stain the plasma membrane (red), and visualized by wide-field microscopy.

(B) ASCs were differentiated into adipocytes and stained with DAPI to visualize the DNA (blue), BoDIPY to stain lipids (green), and an antibody to visualize C/EBPα (red).

(C) Hierarchical clustering and heatmap representation of transcriptional profiles (mRNA) from ASCs, ASC adipocytes, and primary adipocytes. Probe sets are colored according to expression level.

(D) Gene expression of IRF1 in ASCs (n = 3), ASC adipocytes (n = 3), and primary adipocytes (n = 4).

(E) Gene expression of IRF1 in isolated subcutaneous adipocytes of lean (n = 10) and obese (n = 10) individuals.

For all graphs, error bars represent SD, experiments were performed in biological triplicates and statistically significant p values are denoted by asterisks (∗p ≤ 0.05, ∗∗p ≤ 0.005).