Figure 3.

IRF1 Expression Impairs Adipocyte Metabolism and Affects Lipid Composition

(A) Multiplex ELISA assay was utilized to measure cytokines secreted into medium by IRF1 adipocytes compared with control adipocytes.

(B) Glycerol release into the medium in response to forskolin (FSK)-activated lipolysis was measured in IRF1 and rtTA adipocytes.

(C) Radiolabeled glucose uptake was measured in response to insulin.

(D) After 5 days of IRF1 expression, IRF1 adipocytes become unilocular. Lipid droplet size was measured after staining with the lipid dye BoDIPY (green) and the nuclear dye DAPI (blue). CPM, counts per minute.

(E) Total intracellular triglycerides (TG) were measured in IRF1 and rtTA adipocytes.

(F) Tandem mass spectrometric profiles of triglyceride composition for both IRF1 and rtTA adipocytes.

Error bars represent SD, experiments were performed in biological triplicates, and statistically significant p values are denoted by asterisks (^∗p ≤ 0.05, ^∗∗p ≤ 0.005). n = 3 for each experiment.