Figure 4.

IRF1 Leads to Increased Inflammation In Vivo

(A) 3T3-F442A cells were transduced with either rtTA alone, or human IRF1 (hIRF1) containing lentiviral particles, and were injected into 6-week-old nude mice (n = 4). Six weeks after injection, ectopic subcutaneous fat pads (dashed circles) were excised.

(B) Ectopic fat explants (EXP) were stained with DAPI (blue) and probed with α-F4/80 (green) and α-PLIN1 (red).

(C) F4/80-positive cells were counted relative to DAPI staining foci in hIRF1 explants versus rtTA explants and autologous WAT (see Figure S3C). A minimum of 10⁴ DAPI foci were counted for each experiment.

(D) qPCR was used to assess expression of hIRF1 and inflammation-associated mouse genes in both explants and autologous WAT (n = 3).

Error bars represent SD, experiments were performed in biological triplicates, and statistically significant p values are denoted by asterisks (^∗p ≤ 0.05, ^∗∗p ≤ 0.005).