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. 2017 Apr 13;8(5):1242–1255. doi: 10.1016/j.stemcr.2017.03.008

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Specific shRNA Knockdown of PPARγ-Related Candidate Genes Ameliorates Repopulation Capacity of Fancd2^−/− LSKs

(A) Real-time qPCR validation of shRNA efficiency in transduced LSK cells for the different constructs designed to target the indicated genes. Values are presented as mean ± SD. ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(B–D) Isolated LSKs from Fancd2^+/+ or Fancd2^−/− mice (n = 6 donors/group, two independent experiments) were transduced with a specific shRNA construct or scrambled shRNA control. The transduction efficiency was 25%–33% before transplantation and was normalized to 1. Transduced cells along with untransduced cells were transplanted into lethally irradiated Boy/J (n = 8 mice/group, two independent experiments). The graphs indicate relative changes in GFP⁺ proportion among donor-derived blood cells (CD45.2) at different time points after transplantation (B and C), or 4 weeks of serial rounds of transplantations (D). Values are presented as mean ± SD.