Figure 2.

In Vivo Differentiation with the Teratoma Assay

(A) Schematic of the experimental procedure for teratoma induction.

(B) Days of xenograft growth until harvest (±SEM). For each cell line the same batch of cells were injected into six to eight mice (n = 7 except for H9Hyb, n = 8 and hEC, n = 6).

(C) Representative sections of H&E-stained xenografts. Arrowheads indicate derivatives of mesoderm (MES), ectoderm (ECT), and endoderm (END). Scale bars: 200 μm.

(D) IF staining using antibodies against βIII-tubulin (ectoderm), α-fetoprotein (AFP, endoderm), PECAM-1 (mesoderm), OCT3/4, and NANOG (undifferentiated cells). Insets: overlay of OCT3/4 and NANOG. Nuclei were stained with DAPI. Scale bars, 75 μm.

See also Figure S2.