Figure 1.

Inflammatory Activity Regulates Mmp2 and Mmp9 Expression in the VDA

(A) runx1, mmp2, and mmp9 expression was enhanced following exposure (36–42 hpf) to CAY10397 (10 μM) but decreased by indomethacin (10 μM), as determined by qPCR (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; 40 pooled embryos/condition × 3 replicates).

(B) CAY10397 (12–36 hpf) increased mmp2 and mmp9 expression, while indomethacin (Indo) diminished expression by WISH (n ≥ 20/condition).

(C) WISH time course (18–72 hpf) for mmp2 and mmp9 expression (n value as in B).

Arrowheads mark mmp9⁺ cells; brackets mark dorsal/ventral boundaries of the VDA and CV. Scale bars, 100 μm.