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. 2017 Apr 20;8(5):1270–1286. doi: 10.1016/j.stemcr.2017.03.017

Figure 2.

Figure 2

RSV Hinders the Neural Differentiation of mESCs and Induced EpiSCs In Vitro

(A and B) RSV (15 μM) treatment severely compromises the production of GFP+ neural-lineage cells from mESCs, as shown by microscopy (A) and FACS (B) analysis in day-5 SFEBs.

(C) N-CADHERIN and GFP immunostaining of the cells in (A).

(D) RSV inhibits the expression of neural-lineage genes during the neural differentiation of mESCs.

(E) Schematic representation of the generation of induced EpiSCs and targeted neural differentiation in vitro.

(F) qPCR analysis of inner-cell-mass and epiblast markers between mESCs and induced EpiSCs.

(G and H) RSV treatment (15 μM) severely compromises the production of neural-lineage cells from induced EpiSCs, as shown by microscopy (G) and FACS (H) analysis in day-3 SFEBs from induced EpiSCs.

(I) RSV inhibits the expression of neural-lineage genes during the neural differentiation of induced EpiSCs.

(J) N-CADHERIN and GFP immunostaining of the cells in (G).

Ctrl, control (and similarly hereafter). Data are shown as means ± SEM of three independent experiments. Unpaired two-tailed Student's t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus the control. Scale bars, 100 μm.