Figure 1.

iPS-DCregs Suppress T Cell Proliferative Responses In Vitro and In Vivo

(A) Addition of iPS-DCregs suppresses T cell proliferation in allo-MLR (left panel) and OVA-specific MLR (right panel) (n = 3–5 in each group, mean ± SD, pooled from three independent experiments). The details of these two reaction systems are shown in Figure S5. Proliferation of T cells was determined by Violet dilution gated on the CD4⁺ and/or CD8⁺ population. Gray lines are T cells without BM-DCcons stimulation. Statistical analysis was determined by one-way ANOVA and Tukey's test. ^∗p < 0.05, ^∗∗p < 0.01.

(B) Schematic of PLNA protocol.

(C) Addition of iPS-DCregs suppresses T cell proliferative responses in PLNA (n = 3 in each group, mean ± SD, pooled from three independent experiments). Proliferation of T cells was determined by carboxyfluorescein diacetate succinimidyl ester (CFSE) or Violet dilution gated on CD4⁺ (OT-II) or CD8⁺ (OT-I) population. Gray lines indicate PBS injection into the footpad, used as a negative control. Statistical analysis was determined by one-way ANOVA and Tukey's test. ^∗p < 0.05, ^∗∗p < 0.01.