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. 2017 Apr 20;8(5):1174–1189. doi: 10.1016/j.stemcr.2017.03.020

Figure 2.

Figure 2

Administration of iPS-DCregs Generates Tregs In Vivo

(A) CBA mice were immunized with four types of B6 DCs (BM-DCcons, iPS-DCcons, BM-DCregs, iPS-DCregs) on day −7 and were euthanized on day 0. SPCs were harvested for FCM and one-way MLR (secondary immunization).

(B) The percentage of CD25+ FOXP3+ cells in the spleen from iPS-DCregs-immunized CBA was significantly increased compared with other groups (n = 4 in the PBS control group, n = 3 each in other groups, mean ± SD, pooled from three independent experiments). Statistical analysis was determined by one-way ANOVA and Tukey's test. ∗p < 0.05.

(C) T cells from B6 iPS-DCregs immunized with CBA were co-cultured with B6 BM-DCcons for 3 days. Proliferation of Teffs was determined by Violet dilution gated on CD8+ or CD4+ FOXP3− population (n = 3 in each group, mean ± SD, pooled from three independent experiments). Statistical analysis was determined by one-way ANOVA and Tukey's test. No statistically significant differences were observed between these groups.