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. 2017 Apr 20;8(5):1174–1189. doi: 10.1016/j.stemcr.2017.03.020

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© 2017 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

iPS-DCreg Immunization Increases Activated Tregs in Allografts

(A) Allografts were harvested on POD7 and POD14, and were stained with anti-FOXP3 (blue), collagen IV (yellowish-brown), and BrdU (red). The FOXP3⁺BrdU⁺ cells are shown in purple (indicated by the black arrows). Naive B6 hearts served as the control.

(B) The infiltrating lymphocytes in the grafts were separated and triple stained for CD4/CD25/FOXP3 or CD4/CD25/CTLA-4 or CD4/CD25/GITR (n = 5 in PBS-POD7 group, n = 3 in iPS-DCregs POD7 and iPS-DCregs POD14 group; mean ± SD, pooled from three independent experiments). Statistical analysis was determined by one-way ANOVA and Tukey's test. ^∗p < 0.05.

(C) The mRNA expression of FOXP3 in allografts harvested on POD7 and POD14 was detected by qRT-PCR (n = 9 in PBS-POD7 group, n = 6 in iPS-DCregs POD7 group, n = 5 in iPS-DCregs POD14 group; mean ± SD, pooled from five independent experiments). Statistical analysis was determined by one-way ANOVA and Tukey's test. ^∗p < 0.05, ^∗∗∗p < 0.001.