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Typical time course of polarized intensity of contracting psoas muscle myofibril. This is a bar plot, where the vertical scale is the number of counts during 10 msec. Ch1 (black) and Ch 2 (red) are the fluorescence intensities polarized perpendicular (I┴) and parallel (I║) to the myofibrillar axis, respectively. The direction of excitation polarization is ║ to the myofibrillar axis. Laser power = 0.1 μW
