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Kinetics of orientation change of rigor lever arms of WT (a) and MUT (b) R145W myofibrils. The black lines are best fits for WT and MUT muscles. The fluctuations are caused by change of orientation of lever arms only. The other possible source of fluctuations, i.e., fluctuations in the number of fluorophores in the DV (Magde et al. 1974; Elson and Magde 1974) are not significant because PF is only sensitive to rotations and myofibrils were not twisting
