Fig. 5.
Fast closure of the AMPbind loop in AK refolding ensemble. Intra-molecular distance distribution between residues 71 and 28 during refolding and at equilibrium. The broken black line indicates the distance distribution under denaturing conditions (2M Guanidinium-HCl) measured by the double kinetics method. The mean and full width at half maximum FWHM in the denatured state are 38 Ǻ and 34 Ǻ, respectively. The blue and black solid lines mark the 5 ms transient distribution, and the distribution at 5 min after mixing, respectively. The overlap of all the distributions obtained during the refolding transition from 5 ms to 5 min provides solid evidence for very fast closure of the loop structure (reproduced with permission)