Figure 3.
MSCs and MSC-derived EVs Prevent Development of EAU in Mice
(A) Experimental scheme. On day 0, EAU was induced by subcutaneous IRBP injection and intraperitoneal pertussis toxin injection. Right after induction, either MSCs (1 × 106 cells) or MSC-derived EVs (30 μg containing 15 × 109 EVs) were injected into the tail vein. As a control, the same volume of PBS was injected. On day 21, the eyeballs and draining cervical lymph nodes were collected for assays.
(B) Representative microphotographs of H&E staining of the eyes (100× magnification), and histologic disease scores of retinal pathology.
(C) Representative microphotographs of CD3 immunostaining of the eyes (100× magnification), and quantitative data of the number of CD3+ cells infiltrating the retina and vitreous cavity.
Dots represent a single animal, and the data are presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 by one-way ANOVA.