Figure 6.

G9a Directly Methylates DNMT3A2, DNMT3B, and DNMT1

(A) In vitro G9a methyltransferase assay shows that G9a methylates DNMT3A2, DNMT3B, and DNMT1. G9a also methylates DNMT3A2, an alternative splicing form, which lacks the K44 residue.

(B–D) In vitro G9a methyltransferase assay shows that G9a methylates (B) the region of amino acids 126–250 in DNMT3A2, (C) the region of amino acids 241–340 in DNMT3B, and (D) lysine residues at the consensus RK methylation site in DNMT3A2 and DNMT3B. The red asterisks are the methylated proteins.

(E) A schematic diagram of potential sites for methylation by G9a in DNMT3A, DNMT3A2, and DNMT3B.