Figure 1.

Endoglin Is Expressed in CDCs and Can Be Efficiently Depleted Using Cre-LoxP Recombination

(A) Flow-cytometric analyses of murine CDCs at passage 2. Summary of the percentage of CDCs expressing mesenchymal (CD90, endoglin), hematopoietic (CD45), and stem cell (cKit, Sca-1) markers from three independent experiments and plotted as mean percentage ± SEM.

(B) Summary of the floxed endoglin allele (Eng^fl) and Cre recombination driven by the R26-Cre^ERT2 allele in the presence of 4-hydroxytamoxifen to generate a null endoglin knockout (Eng^KO) allele.

(C) Immunostained CDCs showing that transient 48-hr treatment with 4-hydroxytamoxifen leads to efficient endoglin protein depletion. Scale bar, 50 μm.

(D) qPCR analysis showing loss of endoglin transcripts in Eng^KO CDCs. ^∗∗∗p < 0.001.