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. 2017 May 9;8(5):1287–1298. doi: 10.1016/j.stemcr.2017.04.015

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Endoglin Is Required to Maximize Pro-proliferative and Pro-migratory Endothelial Cell Responses

(A and B) Proliferation of HUVECs measured using a fluorometric assay (CyQUANT) at 24 hr (A) and 48 hr (B) is reduced in the presence of Eng^KO CM compared with control CM. Data from nine independent experiments are represented as mean ± SEM; ^∗p < 0.05.

(C and D) Viability of HUVECs was measured using a colorimetric (MTT) assay and is similar following treatment with control CM or Eng^KO CM for 24 hr (C) and 48 hr (D). Data from seven independent experiments are represented as mean ± SEM.

(E and F) HUVEC migration in the scratch-wound assay is significantly reduced in the presence of Eng^KO CM compared with control CM. Data from five independent experiments are plotted as mean ± SEM, ^∗∗p < 0.01.