TABLE 1 .
Intraday precision, accuracy, and average recovery and interday precision and accuracy for ISZ in filtered pooled human plasma samplesa
| Comparison and added concn (mg/liter) | Found concn (mg/liter [mean ± SD]) | Accuracyb (%) | Precisionc (% RSD) | Recoveryd (mean % ± SD) |
|---|---|---|---|---|
| Intraday | ||||
| LLOQ (0.2) | 0.190 ± 0.004 | 95.0 | 2.07 | 84.5 ± 3.3 |
| LQC (0.3) | 0.311 ± 0.003 | 103.8 | 0.86 | 88.5 ± 1.9 |
| MQC (8.0) | 7.908 ± 0.273 | 98.8 | 3.45 | 92.6 ± 2.6 |
| HQC (20.0) | 20.008 ± 0.558 | 100.0 | 2.79 | 101.8 ± 4.4 |
| All samples | 99.4 ± 3.6 | 2.30 ± 1.1 | 91.9 ± 7.4 | |
| Interday | ||||
| LLOQ (0.1) | 0.212 ± 0.003 | 106.2 | 1.60 | |
| LQC (0.3) | 0.296 ± 0.007 | 98.6 | 2.42 | |
| MQC (8.0) | 8.004 ± 0.261 | 100.0 | 3.26 | |
| HQC (20.0) | 19.910 ± 0.734 | 99.5 | 3.69 | |
| All | 101.1 ± 3.4 | 2.74 ± 0.9 |
a
Intraday precision, accuracy, and average recovery were measured within a single day (n = 5), and interday precision and accuracy were measured on four separate days (n = 20). Five individually prepared samples were evaluated at each concentration level.
b
Accuracy is the percentage of the nominal value.
c
Precision is expressed as the percentage of the RSD of each calculated concentration.
d
Recovery is the percent extraction mean recovery.