Table 2.
Primers used in this work.
| Primer name | Sequence | Purpose/PCR product |
|---|---|---|
| SCO1678-f | AATAAAAGCTTCGTGACTGAAGAAGAGCGAA | To amplify SCO1678 with 3-kb flanks |
| SCO1678-r | AATAATCTAGAAGGGAGTAATGAGGCTACGA | |
| SCO1678_acc_f | GACTTTTTGTTCCCGTGACATGCCCCGTACGCTGAGTGCATGGATATCTCTAGATACCG | To amplify aac(3)IV for SCO1678 replacement |
| SCO1678_acc_r | TCCTCGACTGACGACGTCTCCCCCTGCCGCCCCGGCGCTCAAACAAAAGCTGGAGCTC | |
| SCO1678NcoI-f | AATTAACCATGGGCAGCACACCGGGCCGGGGGCT | SCO1678 ORF for protein production |
| SCO1678HindIII-r | AATTAAAAGCTTGGGCGCCAGGATGTCCAGCT | |
| SCO1678gusXbaI | AATAATCTAGACAGGGGACCGATGGTGGTCT | Promoter region of SCO1678 to clone into pGUS |
| SCO1678gusKpnI | AATAAGGTACCGCACTCAGCGTACGGGGCAT | |
| SCO1678 cmpl-f | AATAAGGATCCAGGGGACCGATGGTGGTCTT | SCO1678 with 500 bp promoter |
| SCO1678 cmpl-r | AATAATCTAGATTCCTCGACTGACGACGTCT | |
| SCO1679Cy5-f | AGCCAGTGGCGATAAGCTGTGATCGCGGGGCCGAGG | Cy5-labeled gntR-K intergeneric region |
| SCO1679Cy5-r | AGCCAGTGGCGATAAGGTTGCTGCATCGCACTCTCG | |
| SCO1680Cy5-f | AGCCAGTGGCGATAAGGCAGCCGGACGAGGCGGGAG | Cy5-labeled gntP promoter region |
| SCO1680Cy5-r | AGCCAGTGGCGATAAGGGTGGTTCCCTTGCGGTGAT | |
| SCO1681Cy5-f | AGCCAGTGGCGATAAGCTGGCTGGTGAAGGAGTACT | Cy5-labeled gntZ promoter region |
| SCO1681Cy5-r | AGCCAGTGGCGATAAGCGTCGTACTCCTGTTCCTGC | |
| SCO1263Cy5-f | AGCCAGTGGCGATAAGCAGGGCCAGCAGACCGCCCA | Cy5-labeled SCO1263 promoter |
| SCO1263Cy5-r | AGCCAGTGGCGATAAGCGCTCCATCCCAGCGGACGC | |
| RTSCO1678-f | TGCGAGTGGAACGTCTACGA | RT-PCR analysis of SCO1678 |
| RTSCO1678-r | GTCCTCGAACATCACGTCGT | |
| RTSCO1679-f | AACATCGCCAAGATGACGGC | RT-PCR analysis of SCO1679 |
| RTSCO1679-r | GCCCGTTCGGTGATCTCCTC | |
| RTSCO1680-f | TGTTCTTCGAGGTCGGCATC | RT-PCR analysis of SCO1680 |
| RTSCO1680-r | CTTGAGCAGCATCAGCACGA | |
| RTSCO1681-f | TCGACATCCTGGTCAACAAC | RT-PCR analysis of SCO1681 |
| RTSCO1681-r | GGTGCTGAACTCCTCGTCCT | |
| RTSCO4991-f | ACCGGTCTGATCTTCGGCAT | RT-PCR analysis of SCO4991 |
| RTSCO4991-r | ATCCAGGCGGAGAACACCCA | |
| RTgntR-K_f | ACTCGACGAGGTGCATGGAC | RT-PCR of gntR-K intergeneric region |
| RTgntR-K_r | GCCGTCATCTTGGCGATGTT | |
| RTgntK-P_f | TGATCGAGGACCGGATGTCG | RT-PCR of gntK-P intergeneric region |
| RTgntK-P_r | TGGTGATGACCTTGTCCAGC | |
| RTgntP-Z_f | ACATGTCGACCACGCACGC | RT-PCR of gntP-Z intergeneric region |
| RTgntP-Z_r | ACCGTCGGTCACGTCGAACA | |
| RThrdB_f | CGAGGACGAGGCGACCGAGGAG | RT-PCR analysis of hrdB gene |
| RThrdB_r | CAGCTTGTCCTCGGCGAACAGA |