Figure 1.

Icariin have no cytotoxicity in NP cells and had a significant protective effect on NP cells exposed to H₂O₂ at the concentration of 200 uM and this protective effect was attenuated by the PI3K/AKT pathway inhibitor LY294002. (a) H₂O₂ led to obvious cell death at the concentration of 200 uM to 600 uM. Results are presented as mean ± SD (^∗∗p < 0.01 versus control group). (b) Icariin alone has no effect on the viability and proliferation of NP cells at concentrations up to 50 μM. (c) Icariin protects NP cells against H₂O₂-induced toxicity (^##p < 0.01 versus control group, ^∗∗p < 0.01 versus H₂O₂ group).