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. Author manuscript; available in PMC: 2017 May 11.
Published in final edited form as: J Neurosci Methods. 2014 Oct 12;239:65–74. doi: 10.1016/j.jneumeth.2014.10.001

Fig. 4.

Fig. 4

Injection of collagen into the cerebral vasculature causes anatomic brain injury and behavioral deficits in the mouse. Focal cerebral ischemia was induced by transient filament-occlusion of the middle cerebral artery or by injecting six 1-μg boluses of collagen into the distal right internal carotid artery of the mouse. After 48 h, cerebral infarct volume was quantified by staining brain slices with triphenyltetrazolium chloride (TTC) and neurobehavioral deficit score was assessed. (A) Representative TTC-stained coronal brain sections from the mouse filament-occlusion model (top) and collagen injection model (bottom). (B) Cerebral infarct volumes for the filament-occlusion (solid bar) and collagen injection (open bar) models. (C) Neurobehavioral deficit scores for the filament-occlusion (solid bar) and collagen injection (open bar) models. N = 5 filament occlusion; N = 8 collagen injection.