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. Author manuscript; available in PMC: 2017 May 11.
Published in final edited form as: J Neurosci Methods. 2014 Oct 12;239:65–74. doi: 10.1016/j.jneumeth.2014.10.001

Fig. 5.

Fig. 5

Effect of serial vs. single injection of collagen on cerebral blood flow (CBF), anatomic brain injury and behavioral deficit in the mouse. (A) Laser-Doppler flowmetry was used to monitor CBF in the territory of the MCA before, during, and after serial injections of collagen (6× 1 μg collagen) or a single injection of collagen (5 μg collagen) through a catheter threaded into the distal internal carotid artery. “Base” represents stabilized CBF at baseline expressed as percent; “start” represents first collagen injection; “reperfusion” represents removal of the catheter and release of the the ipsilateral common carotid ligature. After 48 h, cerebral infarct volume was quantified by staining brain slices with triphenyltetrazolium chloride (TTC) and neurobehavioral deficit score was assessed. (B) Cerebral infarct volumes after serial injections of collagen (6× 1 μg; solid bar) or single injection of collagen (1× 5 μg; open bar). (C) Neurobehavioral deficit scores after serial collagen injection (6× 1 μg; solid bar) or single collagen injection (1 × 5 μg; open bar). N = 5 for 5 μg group; N = 10 for 6× 1 μg group.