Figure 5.

Ovariectomy-induced bone loss. Female Igfbp4^+/+ and Igfbp4^−/− mice ovariectomized at 8 weeks of age RNA and killed at 16 weeks of age. (A) RNA was isolated from femurs and subjected to quantitative PCR to observe Igfbp4 gene expression. (B and C) Animals were subjected to DXA at 16 weeks of age to observe bone parameters. aBMC, areal bone mineral content; aBMD, areal bone mineral density. (n = 5–8/surgery group/genotype, *two-way ANOVA P value <0.05.)