Figure 7.

Primary cultures of BMSCs. BMSCs differentiated into osteoblasts were isolated from long bones from 8-week-old Igfbp4^−/− and Igfbp4^+/+ females (A and B) and males (C and D). Osteoblasts were stained for alkaline phosphatase in pink and Von Kossa was performed to show mineralization in black (A and C). RNA was isolated from culture wells in order to examine gene expression of Runx2 and Bglap (B and D). (n of mice = 3/gender/genotype/experiments, n of wells = 3/gender/genotype.)