Figure 8.

Primary cultures of HSCs. HSCs differentiated into osteoclasts were isolated from long bones from 8-week-old Igfbp4^−/− and Igfbp4^+/+ females and males. (A and C) HSCs were differentiated into osteoclasts for 14 days in 24-well plates. Osteoclasts were counted in each culture well as large TRAP-positive purple cells. (B and D) Representative pictures of resorbed area (white) by osteoclasts cultures on a mineral surface labelled by Von Kossa (black). (n of mice = 3/gender/genotype/experiments, n of wells = 6–7/gender/genotype, **t-test P value <0.01.)