Table 1.

Histomorphometry of Igfbp4^−/− mice.

	Females			Males
	Igfbp4+/+	Igfbp4−/−	t-Test	Igfbp4+/+	Igfbp4−/−	t-Test
MS/BS (%)	38.7 ± 1.77	36.21 ± 1.31	0.076	41.16 ± 2.74	28.46 ± 3.59	0.049
MAR (µm/day)	1.82 ± 0.02	1.63 ± 0.07	0.037	1.04 ± 0.06	0.93 ± 0.04	0.215
BFR (µm3/µm2/day)	0.68 ± 0.06	0.59 ± 0.04	0.216	0.41 ± 0.04	0.30 ± 0.03	0.062
Ob.S/B.Pm	12.77 ± 1.21	16.10 ± 2.10	0.207	2.96 ± 0.81	1.68 ± 0.35	0.139
N.Ob/B.Pm	8.72 ± 0.83	10.92 ± 1.31	0.173	2.24 ± 0.64	1.66 ± 0.31	0.405
OS/BS µ	9.21 ± 1.38	7.81 ± 1.42	0.485	11.03 ± 1.31	4.28 ± 1.09	0.003
O.Th	2.58 ± 0.12	2.59 ± 0.12	0.158	2.36 ± 0.19	2.11 ± 0.13	0.308
Oc.S/BS	12.3 ± 0.79	14.97 ± 0.60	0.022	6.99 ± 0.46	6.69 ± 0.92	0.782
N.Oc/B.Pm	4.24 ± 0.18	5.05 ± 0.21	0.016	2.95 ± 0.17	2.77 ± 0.31	0.624
ES/BS	3.96 ± 0.35	6.38 ± 0.99	0.048	1.23 ± 0.37	1.90 ± 0.45	0.282
N.Ad/T.Ar	42.73 ± 8.16	50.12 ± 4.19	0.482	5.13 ± 2.29	5.79 ± 2.94	0864

Static and dynamic histomorphometry analysis of proximal tibia labeled with calcein and demeclocycline from Igfbp4^−/− mice compared to littermate controls at 16 weeks of age. Bold values represent measurements for which the difference from littermate gender-matched controls reach statistical significance.

BFR/BS, bone formation rate/bone surface; ES/BS, erosion surface/bone surface; MAR, mineral aposition rate; MS/BS, mineralized surface/bone surface; N.Ad/T.Ar, number of adipocyte/total area; N.Ob/B.Pm, number of osteoblast/bone perimeter; N.Oc/B.Pm, number of osteoclast/bone perimeter; Ob.S/B.Pm, osteoblast surface/bone perimeter; Oc.S/BS, osteoclast surface/bone surface; OS/BS, osteoid surface/bone surface; O.Th, osteoid thickness. (n = 5–6/genotype/gender, t-test P values were considered statistically different when P value <0.05.)