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. 2017 May 11;16:81. doi: 10.1186/s12934-017-0698-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Screening of mutants with better thermostability (→). After being treated at 60 °C for 55 min, the clones were overlayed with 0.3 mg/mL α-naphthyl acetate and 1.3 mg/mL Fast Blue B. Clones with enhanced thermostability (M1) rapidly turned deep brown around the colony