Fig. 6.

Effect of temperature on the activity of the wild-type enzyme (filled square), M1 (filled circle), M2 (filled triangle), and M3 (open square). The optimum temperature was determined analogously by measuring esterase activity in the temperature range of 35–80 °C in a phosphate buffer (50 mM, pH 6.5). Data points are the average of triplicate measurements, and error bars represent the standard deviation