Table 2.
Unique substitutions in isolates from the EV-D68 B1 subclade in comparison with non-B1 EVD68 and their relationship with residues in PV, EV-D70, and EV-A71 viruses
| EV-D68 genome region | Position (UTR: NT; mature peptides: AA)a | B1 EV-D68 NT/AA distribution | Non-B1 EV-D68 NT/AA distribution | P valueb | B1 predominant NT/AA | Sensitivity (%) | Specificity (%) | PV-1/2/3 position and residuec | EV-D70 position and residue | EV-A71 position and residue | Observed in Other Viruses | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5′UTR | 127 | 10 G, 89 T | 17 A, 5 G | 6.25E−03 | T | 90 | 100 | ^ | – | 128 | T | ^ | – | D70 |
| 5′UTR | 188 | 90 A, 23 T | 47 C, 17 T | 2.13E−02 | A | 80 | 100 | 185 | A | 190 | A | 189 | A | PV, D70, A71 |
| 5′UTR | 262 | 91 C, 24 T | 65 T | 2.13E−02 | C | 79 | 100 | 259 | C/T | 264 | T | 264 | A | PV |
| 5′UTR | 280 | 114 C, 1 T | 8 C, 58 T | 6.97E−03 | C | 99 | 88 | 277 | C/T | 282 | T | # | – | PV |
| 5′UTR | 339 | 21 C, 94 T | 65 C, 1 T | 1.95E−02 | T | 82 | 98 | 337 | C/T | 341 | A | 338 | C | PV |
| 5′UTR | 496 | 3 A, 112 G, 1 T | 58 A, 10 G | 1.12E−02 | G | 97 | 85 | 494 | A | 498 | A | 496 | A | |
| 5′UTR | 669 | 92 C, 24 T | 1 C, 54 T | 2.19E−02 | C | 79 | 98 | # | – | # | – | # | – | |
| 5′UTR | 697 | 1 A, 93 C, 22 T | 36 A, 1 C, 18 T | 2.03E−02 | C | 80 | 98 | # | – | # | – | # | – | |
| VP2 | 222 | 22 M, 96 T | 17 M, 18 V | 1.22E−02 | T | 81 | 100 | 243 | S | 224 | T | 228 | A | D70 |
| VP3 | 24 | 96 A, 21 V | 35 V | 1.10E−02 | A | 82 | 100 | 24 | A | 24 | A | 24 | I | PV, D70 |
| VP1 | 98 | 155 A, 4 T | 31 A, 181 T | 1.60E−03 | A | 97 | 85 | # | – | 98 | I | # | – | |
| VP1 | 290 | 27 N, 132 S | 1 D, 211 N | 9.58E−04 | S | 83 | 100 | # | – | 292 | T | # | – | |
| VP1 | 308 | 134 N, 25 T | 8 N, 204 T | 1.61E−03 | N | 84 | 96 | 300 | T | 312 | T | 296 | T | |
| 2A | 66 | 21 D, 101 N | 32 D | 7.50E−03 | N | 83 | 100 | 68 | R | 66 | N | 69 | R | D70 |
| 2C | 1 | 97 G, 20 S | 32 S | 9.36E−03 | G | 83 | 100 | 1 | G | 1 | S | 1 | S | PV |
| 2C | 34 | 1 I, 1 N, 115 T | 26 N, 6 T | 2.35E−03 | T | 98 | 81 | 34 | D/E | 34 | T | 34 | E | D70 |
| 2C | 102 | 117 V | 17 A, 1 I, 14 T | 2.75E−04 | V | 100 | 100 | 102 | Q | 102 | S | 102 | Y | |
| 2C | 273 | 3 D, 107 G, 7 S | 26 D, 5 G, 1 N | 8.74E−03 | G | 91 | 84 | # | – | 273 | K | # | – | |
| 3D | 135 | 116 S | 2 S, 30 T | 4.78E−04 | S | 100 | 94 | 139 | K | 135 | T | 139 | S | A71 |
| 3D | 274 | 94 K, 22 R | 32 R | 1.16E−02 | K | 81 | 100 | 278 | K | 274 | R | 279 | K | PV, A71 |
| 3D | 345 | 18 H, 97 Q, 1 Y | 32 H | 9.14E−03 | Q | 84 | 100 | 349 | D | 345 | E | 350 | E | |
#
, Hypervariable region.
^
, Gap. –, Not available.
aEV-D68 numbering is based on US/CO/13-60.
bP value from statistical analysis accounting for evolutionary correlation among isolates.
cPV numbering is based on PV-1 Mahoney NC_002058.