Table 2.

Binary single-marker agreements as positive/negative counts, and corresponding kappa values (distance of mean 40^−∆∆Cq value to marker-specific cutoff is shown for each sample)

	ERBB2			ESR1			PGR			MKI67
Sample	Counts		Distance to cutoff (Cq)	Counts		Distance to cutoff (Cq)	Counts		Distance to cutoff (Cq)	Counts		Distance to cutoff (Cq)
	Negative	Positive		Negative	Positive		Negative	Positive		Negative	Positive
1	40	0	−1.1	0	40	1.5	0	40	3.2	40	0	−3.2
2	40	0	−1.1	0	40	1.7	0	40	1.8	40	0	−0.8
3	0	40	0.6	0	40	1.9	0	40	2.5	0	40	0.8
4	40	0	−1.2	0	40	3.0	0	40	4.6	0	40	0.7
5	40	0	−1.9	0	40	1.6	0	40	5.6	0	40	0.6
6	40	0	−2.9	0	40	1.4	0	40	6.0	0	40	0.6
7	40	0	−3.2	40	0	−4.0	40	0	−3.0	0	40	2.2
8	0	40	1.7	12	28	0.2	0	40	2.9	0	40	1.3
9	0	30	1.3	30	0	−3.2	30	0	−2.3	0	30	1.1
10	0	30	3.1	30	0	−2.0	30	0	−1.7	0	30	1.5
11	30	0	−1.6	0	30	2.1	0	30	4.9	4	26	0.2
12	30	0	−1.8	0	30	2.7	0	30	4.7	0	30	1.1
13	30	0	−1.8	0	30	2.4	0	30	4.6	1	29	0.4
14	30	0	−2.0	0	30	1.4	0	30	3.2	30	0	−0.8
15	30	0	−1.3	0	30	1.1	0	30	1.0	29	1	−1.2
16	29	0	−1.5	0	29	2.5	0	29	2.9	0	29	0.7
17	30	0	−1.1	0	30	1.4	0	30	0.8	0	30	1.1
18	30	0	−1.3	0	30	2.5	0	30	4.2	30	0	−1.2
19	30	0	−1.5	0	30	2.8	0	30	2.8	1	29	0.4
20	30	0	−2.6	30	0	−0.9	0	30	3.3	29	1	−0.6
21	30	0	−2.8	20	10	0.0	0	30	3.4	29	1	−0.5
22	30	0	−3.1	30	0	−3.4	30	0	−3.1	0	30	2.1
23	30	0	−3.1	30	0	−2.3	16	14	0.0	0	30	2.2
24	0	30	1.9	30	0	−5.2	30	0	−3.3	1	29	0.4
kappa Statistic	1.00			0.91			0.94			0.94

Abbreviations: ERBB2 (HER2) Human epidermal growth factor receptor 2, ESR1 (ER) Estrogen receptor, MKI67 Marker of proliferation Ki67, PGR (PgR) Progesterone receptor

Boldface values represent summary of measurements