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Gating strategy for the identification of MDSCs in the peripheral blood of patients. PBMCs were stained with anti-CD3-FITC, anti-CD19-FITC, anti-CD56-FITC, anti-CD33-APC, anti-HLA-DR-PE/Cy7, and anti-CD14-APC/Cy7 Abs. In the cell subset negative for the lineage markers (CD3, CD19, CD56, and CD14) and HLA-DR, MDSCs were identified as CD33+.
