Fig. 2.

Effect of TFP on APAP-induced reactive nitrogen and oxygen formation in freshly isolated hepatocytes. Hepatocytes were incubated with APAP (1 mM), APAP plus TFP (10uM), TFP alone or with media alone (Con) for 0–3 h. (A) 3-NT in proteins, (B) Nitric Oxide (C) Superoxide production, and (D) GSNO in hepatocytes. Significant increase was indicated by * (p ≤ 0.05) from control group. Samples were n = 3 from 3 separate mice and hepatocytes were isolated on 3 different days. The data are presented as mean ±SE.