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. 2017 May 10;9:8. doi: 10.3389/fnsyn.2017.00008

Figure 4.

Figure 4

Newly synthesized brain-derived neurotrophic factor (BDNF)-induced Arc is SUMOylated during LTP consolidation. (A) Time course plots of medial perforant path-dentate gyrus evoked fEPSPs recorded before and after HFS (indicated by arrows). Values are mean ± SEM of the maximum fEPSP slope expressed as percent of baseline. TrkB-Fc or control IgG-Fc (1 μl, 12.5 min, 100 μg) was infused into the dorsal dentate gyrus at 2 h post-HFS (indicated by a bar) and dentate gyrus tissue was collected at 4 h post-HFS (stippled line). TrkB-Fc infusion reverted ongoing LTP maintenance. (B) Bi-directional coimmunoprecipitation of SUMOylated Arc using anti-SUMO1 and anti-Arc antibodies. Enhanced Arc expression and Arc SUMO1ylation in HFS-treated dentate gyrus was reverted to baseline levels by TrkB-Fc infusion. (C) Bar graph shows fold change in Arc SUMOylation state in HFS-treated dentate gyrus compared to contralateral control in TrkB-Fc and IgG-Fc infused rats. Arc immunoprecipitation was performed and the ratio of 65 kDa (SUMO-Arc) to unmodified 50 kDa Arc was calculated. n = 5; Student’s t-test, *P < 0.05.