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. 2017 May 10;9:8. doi: 10.3389/fnsyn.2017.00008

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Copyright © 2017 Nair, Patil, Tiron, Kanhema, Panja, Schiro, Parobczak, Wilczynski and Bramham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced Arc SUMOylation in the synaptoneurosome compartment during LTP in vivo. (A) Representative Arc immunoblot performed in dentate gyrus whole lysate and fractionated synaptoneurosome samples. (B) Fold change in SUMOylated Arc based on the ratio of the upper and lower Arc immunoreactive bands. Two dentate gyri were pooled each experiment. n = 4; Student’s t-test, *P < 0.05. (C) SUMO1immuno-precipitation (mouse monoclonal) followed by Arc immunoblotting confirmed Arc SUMOylation in synaptoneurosomes. Unmodified Arc is also detected in the SUMO1 pellet. (D) SENP1 digestion removed SUMOylated Arc. The heavy band detected in the Arc and SUMO1 immunoprecipitate is absent in SENP1 treated samples. Arc was immunoprecipitated with mouse monoclonal Arc C7 antibody. ^#The polyclonal rabbit Arc antibody (Synaptic Systems) used for immunoblotting detects a spurious band in the lysate that is absent in the Arc pellet. *Bands due to SENP1 enzyme in the blot; right lane shows SENP1 enzyme loaded alone. 6% polyacrylamide gel.