Fig. 2. Relative fluorescence intensity at 796 nm of probe 1 (10 μM) with various species at 37 °C for 30 min in PBS buffer. Lane 1, only probe; lane 2, 100 μM Fe3+; lane 3, 100 μM Zn2+; lane 4, 100 μM H2O2; lane 5, 100 μM ClO–; lane 6, 100 μM NO2 –; lane 7, 100 μM S2O3 2–; lane 8, 100 μM SO3 2–; lane 9, 1 mM Hcy; lane 10, 1 mM Cys; lane 11, 5 mM GSH; lane 12, 1 mM 2,2′-dithiodipyridine; lane 13, 1 mM 2,2′-dithiodipyridine + 100 μM H2S; lane 14, 1 mM cystamine; lane 15, 1 mM cystamine + 100 μM H2S; lane 16, 10 μM lysozyme; lane 17, 10 μM lysozyme + 100 μM dithiodipyridine + 50 μM H2S; lane 18, 10 μM BSA; lane 19, 10 μM BSA + 100 μM dithiodipyridine + 50 μM H2S; lane 20, 100 μM H2S.
